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(S)CARNA5(+)
(S)CARNA5(+)
規(guī)格:
貨期:
編號(hào):B162381
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 (S)CARNA5(+)
商品貨號(hào) B162381
Designations (S)CARNA5(+)
GenBank Number

M14934

Species Cucumber mosaic virus
Depositors JM Kaper
Vector
Construct size (kb): 3.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 0.4000000059604645
Gene product: satellite RNA (CMV-associated RNA 5, CARNA 5)
Insert Size (kb) 0.400
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.4; HindIII--3.4; BamHI--3.4; PstI--3.4; XbaI--3.4.
Contains a BstEII site 68 nt from the 5' end.
A full-length clone.
Oriented such that transcription from the SP6 promoter gives the sense (+) strand, capable of directing protein synthesis.
Classification Cucumovirus
References

Avila-Rincon MJ, et al. In vitro translation of cucumoviral satellites. I. Purification and nucleotide sequence of a cucumber mosaic virus-associated RNA 5 from cucumber mosaic virus strain S. Virology 152: 446-454, 1986.

Avila-Rincon MJ, et al. In vitro translation of cucumoviral satellites. II. CARNA 5 from cucumber mosaic virus strain S and SP6 transcripts of cloned (S)CARNA 5 cDNA produce electrophoretically comigrating protein products. Virology 152: 455-458, 1986. PubMed: 3487880

J M Kaper, personal communication

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