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72A1
72A1
規(guī)格:
貨期:
編號:B163810
品牌:Mingzhoubio

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產(chǎn)品名稱 72A1
商品貨號 B163810
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications The antibody reacts with the 350000/220000 dalton viral envelope glycoprotein.
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with disrupted fixed Epstein-Barr virus particles.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Epstein-Barr virus (EBV)
Cellular Products
immunoglobulin; monoclonal antibody; against Epstein-Barr virus (EBV)
Comments
The antibody reacts with the 350000/220000 dalton viral envelope glycoprotein.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype mouse IgG1
Name of Depositor GJ Hoffman
Deposited As mouse (B cell); mouse (myeloma)
References

Hoffman GJ, et al. Monoclonal antibody against a 250,000-dalton glycoprotein of Epstein-Barr virus identifies a membrane antigen and a neutralizing antigen. Proc. Natl. Acad. Sci. USA 77: 2979-2983, 1980. PubMed: 6248876

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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