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BL3.1 is a B-lymphosarcoma cell line derived in 1990 by irradiation of the bovine B-lymphoblastoid cell line, BL-3.

The BL-3 cell line was isolated from a 3-month-old male Hereford calf.

BL-3 cells were exposed to gamma-irradiation and surviving cells were immunoselected for MHC class I antigen loss.

BL3.1 cells are a major histocompatibility complex (MHC) transcriptional loss variant. The cells exhibit no expression of MHC class I and high expression of MHC class II. Few cell lines express only MHC class II.

Harms JS, Splitter GA. Impairment of MHC class I transcription in a mutant bovine B cell line. Immunogenetics 35: 1-8, 1992. PubMed: 1345904

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.