| 產(chǎn)品名稱(chēng) |
Hs 888.Lu |
| 商品貨號(hào) |
B164762 |
| Organism |
Homo sapiens, human |
| Tissue |
lung |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Osteosarcoma |
| Age |
20 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Applications |
The line was established from apparently normal tissue from a patient who had metastatic osteosarcoma (see ATCC CRL-7622). See also ATCC CRL-7517 CRL-7632, ATCC CRL-7633, ATCC CRL-7634, ATCC CRL-7635, ATCC CRL-7642, ATCC CRL-7643, ATCC CRL-7644 and ATCC CRL-7645 for lines derived from the same patient. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. |
| Derivation |
The line was established from apparently normal tissue from a patient who had metastatic osteosarcoma (see ATCC CRL-7622). See also ATCC CRL-7517 CRL-7632, ATCC CRL-7633, ATCC CRL-7634, ATCC CRL-7635, ATCC CRL-7642, ATCC CRL-7643, ATCC CRL-7644 and ATCC CRL-7645 for lines derived from the same patient. Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. |
| Clinical Data |
The line was established from apparently normal tissue from a patient who had metastatic osteosarcoma (see ATCC CRL-7622). See also ATCC CRL-7517 CRL-7632, ATCC CRL-7633, ATCC CRL-7634, ATCC CRL-7635, ATCC CRL-7642, ATCC CRL-7643, ATCC CRL-7644 and ATCC CRL-7645 for lines derived from the same patient. male Caucasian 20 years |
| Comments |
The line was established from apparently normal tissue from a patient who had metastatic osteosarcoma (see ATCC CRL-7622). See also ATCC CRL-7517 CRL-7632, ATCC CRL-7633, ATCC CRL-7634, ATCC CRL-7635, ATCC CRL-7642, ATCC CRL-7643, ATCC CRL-7644 and ATCC CRL-7645 for lines derived from the same patient. Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. Please see the NBL Repository description. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Passage History |
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. |
