| 產(chǎn)品名稱 |
J.gamma1.WT |
| 商品貨號(hào) |
B164868 |
| Organism |
Homo sapiens, human |
| Cell Type |
T lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
clusters in suspension |
| Biosafety Level |
2 [Cells contain CMV and SV-40 viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
acute T cell leukemia |
| Gender |
male |
| Applications |
The J.gamma1.WT cell line was derived from the phospholipase C-gamma1 (PLC-gamma1) deficient J.gamma1 cell line (ATCC CRL-2678) by stable transfection with a wild-type expression vector, pcaPLC-gamma1. The J.gamma1.WT derivative (ATCC CRL-2679) stably expresses PLC-gamma1at levels comparable to the Jurkat cell line. |
| Derivation |
The J.gamma1.WT cell line was derived from the phospholipase C-gamma1 (PLC-gamma1) deficient J.gamma1 cell line (ATCC CRL-2678) by stable transfection with a wild-type expression vector, pcaPLC-gamma1. The J.gamma1.WT derivative (ATCC CRL-2679) stably expresses PLC-gamma1at levels comparable to the Jurkat cell line. |
| Clinical Data |
male |
| Comments |
The J.gamma1.WT cell line was derived from the phospholipase C-gamma1 (PLC-gamma1) deficient J.gamma1 cell line (ATCC CRL-2678) by stable transfection with a wild-type expression vector, pcaPLC-gamma1. The vector contains cytomegalovirus (CMV) and SV40 viral DNA sequences and the neomycin resistance gene. The J.gamma1.WT derivative (ATCC CRL-2679) stably expresses PLC-gamma1at levels comparable to the Jurkat cell line. |
| Complete Growth Medium |
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.4 mg/ml G418, 90%; fetal bovine serum, 10%
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| Subculturing |
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10 exp5 viable cells/ml. Maintain cell density between 3 X 10 exp5 and 2 X 10 exp6 viable cells/ml. |
| Cryopreservation |
culture medium, 45%; fetal bovine serum, 45%; DMSO, 10% |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
RT Abraham |
| Deposited As |
human |
| References |
Irvin BJ, et al. Pleiotropic contributions of phospholipase C-gamma1 (PLC-gamma1) to T-cell antigen receptor-mediated signaling reconstitution studies of a PLC-gamma1-deficient Jurkat T-cell line. Mol. Cell. Biol. 20: 9149-9161, 2000. PubMed: 11094067
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