| 產(chǎn)品名稱 |
alpha12-Q229L pcDNAI |
| 商品貨號(hào) |
B235851 |
| Designations |
alpha12-Q229L pcDNAI |
| Species |
Mus musculus, mouse |
| Depositors |
HR Bourne |
| Applications |
contains sequence G protein, alpha subunit |
| Vector |
Construct size (kb): 6.0 |
| Insert |
DNA: cDNA Insert lengths(kb): 2.0 Gene product: G protein, alpha subunit Target Gene: G protein, alpha subunit |
| Insert Size (kb) |
2.0 |
| Media |
ATCC® Medium 1951: LB medium (ATCC medium 1065) with 50 mcg/ml ampicillin and 15 mcg/ml tetracycline
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--6.0; HindIII/XbaI--4.0, 2.0; BglII--6.0. Contains G protein alpha 12 with a Q229->L activating mutation. Expresses protein in mammalian cells. T7 promoter is the closest to 5' end. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Voyno-Yasenetskaya T, et al. G alpha 13 stimulates Na-H exchange. J. Biol. Chem. 269: 4721-4724, 1994. PubMed: 8106440
|
